Gram-negative bacteria (GNB) are among the world's most significant public health problems due to their high resistance to antibiotics. These microorganisms have significant clinical importance in hospitals because they put patients in the intensive care unit (ICU) at high risk and lead to high morbidity and mortality.[1][2] Two large groups, …Screw the lid into place, or push down until you hear a soft click if you’re using a snap top tube. Confirm that the lid is secure before moving on. 3. Shake the tube gently to mix the bacterial culture and glycerol. Whisk the tube back and forth a few times until both liquids are evenly distributed.

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